FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell62.35200000095741.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell62.35200000095741.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1804184
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA42670.23650581093724365No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG28380.1573010291633226No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT22800.12637291983522744No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC21790.12077482119340378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG20950.030.819065
TCCGATC41100.030.0505223
CTTCCGA41650.029.8697571
TTCCGAT41900.029.4767672
CCGATCT42350.029.0573124
CGATCTA7150.026.4298555
CGATCTC11800.022.115545
ATCTGCG2900.021.7210887
GACGTGT5100.019.85015510
CGATCTT6850.019.7052615
GATCTGT6250.018.3574226
GCGAACT3350.018.1332928
GATCTGG14900.018.1182616
GAGACGT5400.017.9141528
CGAACTA3400.017.86662329
ACGTGTG5700.017.76066411
ATCTCGT1551.0930307E-817.4169087
GGTACTA4100.017.01640939
GATCTAG4700.016.7529686
ACGGGAC952.8234947E-416.57661814
Produced by FastQC (version 0.11.2)