FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell61.35200000095727.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell61.35200000095727.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1995630
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA59810.2997048551084119No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG39430.1975817160495683No Hit
ATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATAGC35620.17849000065142337No Hit
GCATAATATAGCAAGGACTAACCCCTATACCTTCTGCATAATGAATTAACT29830.1494766063849511No Hit
AGATATAGTACCGCAAGGGAAAGATGAAAAATTATAACCAAGCATAATATA29470.14767266477252797No Hit
GTATAGGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTT23280.11665489093669668No Hit
CCCTATAGAAGAACTAATGTTAGTATAAGTAACATGAAAACATTCTCCTCC22480.11264613179797858No Hit
GTTATATGTTTGGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTC21690.10868748214849445No Hit
GATTTATAGGTAGAGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGT21360.10703386900377324No Hit
CTTCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATA20800.10422773760667056No Hit
ACCTTAGCCAAACCATTTACCCAAATAAAGTATAGGCGATAGAAATTGAAA20330.1018725916126737No Hit
ACATAGACGGGTGTGCTCTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTC19980.10011875948948451No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAAG401.5609801E-533.75050728
CGATCTG19250.028.1643145
CCGATCT38250.027.0544854
TCCGATC38750.027.0537243
CTTCCGA38750.027.0530471
ACGCGTG507.146841E-527.0078540
CGTTCGG507.16393E-526.99837524
TTCCGAT39350.026.6412162
CCGGGTA551.3697204E-424.54274714
CGATCTA5950.023.063535
GACGTGT8900.022.74915910
GACGCGT500.002262025622.50541339
ACGTGTG9550.021.20078811
CGATCTC11650.021.0481265
AGACGTG9350.020.932479
GATCTGG11400.020.9176986
CTGCGTA654.2443283E-420.7659029
GAGACGT9750.020.7659028
GACGATA550.00393034320.46100641
CGATCTT5500.020.0422385
Produced by FastQC (version 0.11.2)