FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell60.3520000009571a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell60.3520000009571a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2188589
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA30290.13839967211751497No Hit
GTATGGATAGCAAATAGTAGCACTGCCACAACTAACTCAATGCATGTGTAA28860.13186578201754645No Hit
CACTTGGAAGTATGCTTCATGTATTCAGATTTTCACTTCATCAATGAGCAA25340.11578236023300856No Hit
GTAGTAAACAGTATTTGCAACACTACAGGGGCTGAGAAACCAAAGTTTCTA25000.11422884790154753No Hit
GTATATGTGAACTTCTCTCCTTGTTACTCCAATTTCGATGAATCTATTCTC23750.10851740550647014No Hit
CACATATACTATCTGGAAAAGGCCAATAAAATTAAATCTGAGAAAACACAC22990.10504484853026311No Hit
TAGTAGCACTGCCACAACTAACTCAATGCATGTGTAAGGAAGGAGTTGAAC22710.10376548543376578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG24500.030.0250155
CTTCCGA50050.029.9344831
TCCGATC51550.028.8016173
TTCCGAT52100.028.497572
CCGATCT52100.028.4543914
CGATCTA8300.026.8323485
ACGTCGA551.3683629E-424.5464928
CGATCTT8700.022.495815
GACGTGT7950.021.78839310
CGTCGAA550.00393669620.45540829
GAGACGT9000.019.4963678
GATCTGT8050.019.2821226
ACGTGTG9500.018.7070411
CGTCGAC851.2023638E-418.53485342
GATCTGC12050.018.2953456
ATATCGT750.001112049117.9966476
GATCTGG15950.017.9120246
CGGGTAT901.8680062E-417.49833715
AGACGTG10100.017.3730019
CGCCGTA1053.1152078E-517.14240525
Produced by FastQC (version 0.11.2)