FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell6.35200000095048.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell6.35200000095048.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1938895
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA20190.10413147694949959No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG17650.031.610875
CTTCCGA36200.031.1340961
TCCGATC36250.030.9686323
CGATCTA5750.030.9092715
CCGATCT36400.030.7174054
TTCCGAT37300.030.1563992
CGATCTC11400.022.4972535
CGATCTT6050.021.5676165
TCTTCCG12600.020.8903085
CCGGTTA706.97461E-419.29032341
GATCTGC9750.018.2285446
TATATCG1002.0499654E-517.9978035
GATCTAC4450.017.1889136
TCTTACG1053.1181607E-517.1403222
CTGTGCG1459.69103E-817.0668819
CGGTACT3100.016.69538538
GGCGTTA2450.016.5349942
GCTCTTC16600.016.5341243
GATCTCG5600.016.0694686
TACACCG1451.8125884E-615.5153475
Produced by FastQC (version 0.11.2)