FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell59.352000000956ff.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell59.352000000956ff.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914996
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA37280.1278903984773907No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA10600.028.4403295
CGATCTG29000.026.6867755
TCCGATC64250.026.6464733
TTCCGAT64950.026.2900162
CCGATCT65100.026.2294394
CTTCCGA66600.025.8075851
GACGTGT18050.023.93093710
ACGTGTG18100.023.36764311
GAGACGT20850.022.7673268
GGGAGAC24350.022.0818086
GGAGACG24150.021.6125767
AGACGTG21600.020.518629
CGATCTT11200.020.0871185
CGTGTGC22150.019.70475812
CGATCTC22800.018.1559355
CCGAATA800.001712736116.87346812
ATCTGCG3950.016.5172067
GTATTAG10600.015.7055851
CGGGAGA7000.015.4269075
CGGGTAT2051.3205863E-915.36472415
Produced by FastQC (version 0.11.2)