FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell58.352000000956d5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell58.352000000956d5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2250921
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA29330.13030221851411045No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA9750.026.3047855
TCCGATC58750.024.7759823
TTCCGAT58850.024.6191962
CGATCTG26050.024.6134015
GACGTGT18750.024.59727910
CCGATCT58800.024.5253494
CTTCCGA59700.024.2681351
GAGACGT19950.024.01998
ACGTGTG19400.023.19331211
GGAGACG22250.023.053637
GGGAGAC22700.022.9930526
CGATCTT9700.022.7294465
CGCGCAT500.002261474322.5065140
AGACGTG21350.021.2857049
CGTGTGC22050.020.40590712
CGATCTC18400.017.7290175
TCTATAC3850.016.9461783
CGGGTAT1501.426306E-716.49817515
CGGTACT3100.015.97094338
GATCTAC6550.015.7997796
Produced by FastQC (version 0.11.2)