FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell57.352000000956bb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell57.352000000956bb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2261032
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23200.028.316575
TCCGATC52450.028.309663
TTCCGAT52750.028.0633562
CCGATCT52250.028.03054
CTTCCGA53400.027.7211481
CGATCTA9700.025.7452455
CGATCTT9650.023.5472345
TATACCG654.242398E-420.7674755
CGATCTC16950.020.3080185
GAGACGT10300.020.0953878
GACGTGT10150.019.5057410
ACGTGTG10300.018.34796111
GTACTAG1802.0918378E-1017.4977471
ACGTCCG800.001708960216.878843
AGACGTG12150.016.6652589
TAAACCG952.8226874E-416.5775455
ATCTGCG3450.016.3029697
GGAGACG13300.016.2392277
GGGAGAC13850.015.919236
GTATTAG7100.015.8430311
Produced by FastQC (version 0.11.2)