FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell56.35200000095692.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell56.35200000095692.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1866780
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG17100.025.2603025
TTCCGAT39000.024.6318282
TCCGATC39300.024.3293083
CGATCTA7400.024.3215735
CCGATCT39350.024.184054
GAGACGT15900.022.9219348
CTTCCGA42500.022.8150651
CGATCTT6250.022.3174745
GACGTGT15900.022.07297310
ACGTGTG16000.021.9350211
GGAGACG17800.021.2335537
GGGAGAC18750.020.9976236
AGACGTG17300.020.4167659
CGAGGGA1009.070973E-720.2477113
CGTGTGC18650.019.90391512
GACGCGT600.006504154318.75390839
TAAGACG851.20599725E-418.5273174
TAAACGC3750.017.39943128
TAATACG1205.1821626E-616.8730934
CGGGTAT1257.639479E-616.19816815
Produced by FastQC (version 0.11.2)