Basic Statistics
Measure | Value |
---|---|
Filename | HT5LJBCXY_l02n02_inf_cell55.35200000095678.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 130165 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 1842 | 1.4151269542503746 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 257 | 0.1974417086006223 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 254 | 0.19513694157415587 | No Hit |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 143 | 0.10986056159489879 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT | 141 | 0.10832405024392118 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ATGCTGC | 20 | 6.9987256E-4 | 45.014988 | 44 |
AGGAGCA | 20 | 7.011996E-4 | 44.9977 | 7 |
ACGCTCT | 20 | 7.011996E-4 | 44.9977 | 11 |
GCTACGT | 20 | 7.011996E-4 | 44.9977 | 8 |
TGTCCGG | 20 | 7.011996E-4 | 44.9977 | 29 |
TCCGTTA | 25 | 3.872877E-5 | 44.997696 | 32 |
TGATCTC | 25 | 3.872877E-5 | 44.997696 | 24 |
CCGAGCT | 35 | 6.207878E-6 | 38.569454 | 23 |
TGTGGAC | 30 | 1.13214504E-4 | 37.512493 | 43 |
GTTCTGC | 30 | 1.13471106E-4 | 37.498077 | 25 |
GGGCTAC | 30 | 1.13471106E-4 | 37.498077 | 6 |
CTATCTC | 30 | 1.13471106E-4 | 37.498077 | 24 |
CAGGGCG | 30 | 1.13471106E-4 | 37.498077 | 33 |
GCCTAGG | 30 | 1.13471106E-4 | 37.498077 | 1 |
GACGACG | 30 | 1.13471106E-4 | 37.498077 | 39 |
ATCTCGT | 105 | 0.0 | 36.426704 | 26 |
GATACGT | 50 | 4.8245056E-8 | 35.998154 | 8 |
TCTCTCA | 25 | 0.0021007934 | 35.998154 | 27 |
CGACGAG | 25 | 0.0021007934 | 35.998154 | 38 |
GGCGTTT | 25 | 0.0021007934 | 35.998154 | 10 |