FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell55.35200000095678.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell55.35200000095678.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130165
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA18421.4151269542503746No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2570.1974417086006223No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2540.19513694157415587No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1430.10986056159489879No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT1410.10832405024392118Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTGC206.9987256E-445.01498844
AGGAGCA207.011996E-444.99777
ACGCTCT207.011996E-444.997711
GCTACGT207.011996E-444.99778
TGTCCGG207.011996E-444.997729
TCCGTTA253.872877E-544.99769632
TGATCTC253.872877E-544.99769624
CCGAGCT356.207878E-638.56945423
TGTGGAC301.13214504E-437.51249343
GTTCTGC301.13471106E-437.49807725
GGGCTAC301.13471106E-437.4980776
CTATCTC301.13471106E-437.49807724
CAGGGCG301.13471106E-437.49807733
GCCTAGG301.13471106E-437.4980771
GACGACG301.13471106E-437.49807739
ATCTCGT1050.036.42670426
GATACGT504.8245056E-835.9981548
TCTCTCA250.002100793435.99815427
CGACGAG250.002100793435.99815438
GGCGTTT250.002100793435.99815410