FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell54.3520000009565f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell54.3520000009565f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2035773
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA20630.10133742809242485No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC38200.029.2684483
CGATCTA6000.029.2448925
CCGATCT38450.028.9026244
CGATCTG17550.028.7129335
TTCCGAT39050.028.688972
CTTCCGA40150.027.902971
CGATCTT7850.023.2125055
GAGACGT6500.021.457798
CGATCTC12100.021.3805645
GACGTGT6250.020.15647910
CGCCTAT1701.8299033E-917.20752736
CTATCGC1752.6921043E-916.71547132
GGAGACG8650.016.6444917
ACGTGTG7150.016.36077711
GTATTAG7850.016.0481511
CGTACGC850.002569067815.879583
AGACGTG8350.015.6260129
TCTTCCG16400.015.5003415
GGGAGAC9900.015.4518466
GTATAAT7750.015.3844091
Produced by FastQC (version 0.11.2)