FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell53.35200000095628.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell53.35200000095628.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758490
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC30800.029.434153
TTCCGAT31750.028.6242942
CCGATCT31650.028.5015054
CGATCTG15750.028.4229855
CTTCCGA32800.027.7765541
CGATCTA4900.024.3319515
CGATCTC9800.021.8069365
CGATCTT6050.021.194185
GACGTGT5750.021.12628410
GAGACGT6000.020.2460218
CGTGTGC6600.019.08715812
ACGTGTG6300.018.5677811
GTATTAC2350.018.1879141
ATCTGCG2250.017.9964627
AGACGTG6400.017.9261639
CCGCTAG3700.017.02900736
CGCTAGA3750.016.80243137
GATCTGC8900.016.6821146
GTCTTAT2450.016.5273631
GCATTCG4350.016.03406722
Produced by FastQC (version 0.11.2)