FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell52.3520000009560e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell52.3520000009560e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1672492
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT29350.028.2843342
TCCGATC29750.027.9040413
CTTCCGA30300.027.4717791
CGATCTA5400.026.66335
CCGATCT31250.026.4206644
CGATCTG15350.026.0879125
AGACGTG5450.023.529149
GACGTGT5550.023.1051910
ACGTGTG6150.021.58264211
GAGACGT6050.020.8238188
CGATCTC10800.019.9974755
CGCACCG600.006511957418.74987629
CCGTACC600.00651420618.74875524
TAATACG1102.2599543E-618.4067674
TCGTTAA2100.018.2125312
ATCTGCG2600.018.1707847
CGAGCCT2600.017.30757729
CGTGTGC7950.017.26248712
TCTTCCG13400.016.6210375
CCGTGAT952.8185308E-416.58032436
Produced by FastQC (version 0.11.2)