FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell51.352000000955e3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell51.352000000955e3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2054761
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5600.029.3250455
TCCGATC32600.028.7754613
TTCCGAT33700.028.1032162
CCGATCT33650.027.810714
CTTCCGA35000.027.1879311
CGATCTG15750.026.2809525
CGATCTT7750.022.931335
TACCGTC2050.020.8498827
GACGTGT7050.020.10274110
ACCGTCG2150.019.880128
ACGTGTG6950.019.4209411
CCGTCGG2550.019.41769840
CGTCGTA2200.018.40575610
CGATCTC10650.017.9544935
GAGACGT8650.017.9447258
GGCGTTA2650.017.8369442
CGTCGGC2900.017.07418441
AGACGTG8000.016.8719439
CCCGTCG2850.016.5836139
CCGTCGT2600.016.439339
Produced by FastQC (version 0.11.2)