FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell50.352000000955c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell50.352000000955c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1368291
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA20720.15142977626835227No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG15280.11167215161102427No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA27700.028.9939561
TCCGATC28200.028.2405513
CCGATCT28000.028.0405454
CGATCTA5000.027.8959225
TTCCGAT28700.027.7485562
CGATCTG14500.026.6857555
CGATCTC7700.023.0810415
CGGTCGA500.002265790222.49835820
ATCGTAC500.002266271522.49753410
GATCTAG2750.021.2696176
GTAATCG754.60605E-520.99846520
CGGTACT2050.020.85594638
TCGCGGT550.003938582320.4530517
GACGTGT2950.019.82833510
GGGGTTA3400.019.1883726
CGATCTT4400.018.9176885
CGCTATA600.0065042518.75342639
CGCGAAT600.006511116418.74999833
TACGACG2050.018.655815
GGTACTA2350.018.19481539
Produced by FastQC (version 0.11.2)