FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell5.3520000009502e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell5.3520000009502e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991117
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25620.12867149444256665No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA39050.032.433611
CGATCTG20250.031.7723185
TCCGATC40150.031.4889813
CGATCTA5600.031.33395
TTCCGAT40750.031.0805452
CCGATCT41150.030.669094
CGATCTC12950.023.7989985
CGATCTT6200.022.8589735
TCTTCCG12400.021.770455
GATCTGC10500.019.2823986
ACGTCCG1751.382432E-1018.00685343
GATCTGG14000.017.9969066
GTATATA6900.017.93171
GATCTCG6200.017.7792026
TTAACGG2800.017.67997435
GCTCTTC15750.017.5684093
GATCTAC3500.017.354166
GTATTAA6250.016.9170911
CGGTACC2950.016.7860545
ACCGCCT3100.016.69151712
Produced by FastQC (version 0.11.2)