FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell49.352000000955a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell49.352000000955a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1392598
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA19450.1396670108674578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA4600.028.8538865
TCCGATC31850.027.6876893
CTTCCGA32200.027.5963291
TTCCGAT32200.027.45662
CCGATCT32900.026.7356624
CGATCTG16850.026.1677445
CGATCTC10200.021.6140425
CGATCTT4250.021.1729375
GACGTGT5400.020.8298610
ACGTGTG6150.019.75280411
GAGACGT6150.019.0212178
CGAACTA1354.2717147E-818.33422729
TCTTCCG13000.017.9969985
GATCTAG2700.017.4970826
AGACGTG6450.017.4389519
GATCTAC3250.017.3048066
CGTGTGC6800.017.20301212
AGACCGT1205.1829065E-616.8721876
ATCTGCG2057.4578566E-1116.460677
GATCTGT5950.016.2577926
Produced by FastQC (version 0.11.2)