FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell48.35200000095586.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell48.35200000095586.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1973229
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA21260.10774218299041824No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTCGA507.152013E-527.00495741
CGATCTG23650.026.4450175
TTCCGAT49650.026.1448842
TCCGATC49600.026.1258853
CCGATCT50050.025.8460354
CTTCCGA51400.025.2985081
CGGTCGA551.3691017E-424.54436320
GAGACGT16250.024.0894058
GACGTGT15450.024.02623210
CGATCTA7650.023.5265775
ACGTGTG15650.022.71349311
GGGAGAC19500.021.3435826
AGACGTG17400.021.3336359
CGATCTT8300.021.142035
GTCGACG654.233016E-420.77409743
GGAGACG19850.020.6272377
GATACGT550.003940272620.452088
CGATCTC15750.020.283275
TGCTACG706.991563E-419.28339210
CGGGAGA6000.019.1226945
Produced by FastQC (version 0.11.2)