FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell47.3520000009556c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell47.3520000009556c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2419466
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG23100.026.1988075
CTTCCGA51300.026.1367861
TCCGATC51600.025.7673663
TTCCGAT51650.025.7424222
CCGATCT52350.025.4411814
CGATCTA9250.024.322015
GAGACGT14800.022.3458488
GACGTGT14100.022.33830310
CGATCTT10300.021.4057315
ACGTGTG14800.021.12974711
TAGACCG550.003939945320.45265
CGTATAC550.00394041720.4521772
AGACGTG16050.019.6243029
CGGTCGA951.3174242E-518.9463520
GGAGACG18200.018.5421947
CGTGTGC18250.017.75173812
GGGAGAC19500.017.5367936
TACCGTC3500.017.3554947
GCGACGT1305.786096E-717.3085537
CGATCTC17300.016.9059075
Produced by FastQC (version 0.11.2)