FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell46.35200000095543.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell46.35200000095543.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214187
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24960.11272760611456938No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC50350.027.299683
CTTCCGA50600.027.298181
CGATCTG23550.027.2251195
TTCCGAT50800.027.057852
CGATCTA7450.026.8750675
CCGATCT50700.026.8449924
CGATCTT9700.021.8007765
GAGACGT11500.021.5184358
GTCGACG754.588144E-521.01006143
ATCTGCG2950.020.590067
GACGTGT11950.019.95509310
CGATCTC15850.019.5868975
ACGTGTG12400.018.323811
GGAGACG14250.018.1551067
GGGAGAC14850.017.8760416
AGACGTG13150.017.6208699
TACCGTC2950.016.7770847
ATACCGT3250.016.6128336
GATCTGG14000.016.390346
CGTGTGC14050.016.17189212
Produced by FastQC (version 0.11.2)