FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell45.35200000095529.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell45.35200000095529.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2530025
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATGTAAGGCTTGCATAAATGTTATTTGCTCAAAACTATTCTCTGTTATCT34030.1345045997569194No Hit
GTGTTATCATTCCATTCAAGTCCCCCGATGAGGACTCCAACTGCATTTTTG32080.12679716603590874No Hit
ATATTAGAGTCTCCAGCCGGTCAAAAATCACACTGAAGTTCGCTTTCAGTA31780.1256114070019071No Hit
TCCAAACACTGTGTCAAGCTTTCAGGTAGATTGCTTTCTTTGGCATGTCCG25810.10201480222527445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG24200.025.8427835
CTTCCGA55550.025.675271
CCGATCT55600.025.571264
TCCGATC55850.025.5373553
TTCCGAT57750.024.7750762
GACGTGT15900.024.19454410
CGATCTA8900.023.5073015
CGGGTAT1152.6557245E-1023.47572515
AGACGTG17050.022.298329
CGATCTT10850.021.563215
GAGACGT19050.021.3743748
CGTGTGC18300.021.02190412
GTCGGAT908.2654005E-619.99783913
ACGTGTG18750.019.67709511
GGAGACG21800.019.2972287
CGATCTC18850.019.0949485
CCGAACG1002.050119E-517.99805613
GCCGCGA2005.0931703E-1116.88185145
ATTACGT800.00171126516.87551133
GCTCTAT5750.016.8232731
Produced by FastQC (version 0.11.2)