FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell44.3520000009550f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell44.3520000009550f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1999328
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22190.11098729173002128No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5650.028.2693565
GTATACG507.169597E-526.9952431
CGATCTG17800.026.6666495
TCCGATC39350.026.0691053
CCGATCT39800.025.8308774
TTCCGAT40200.025.5178932
CTTCCGA41400.024.9955961
GTCGACG602.4617347E-422.50898443
GACGTGT12350.022.40495910
GAGACGT13950.021.7703578
ACGTGTG12750.021.34917811
AGACGTG13600.020.8419159
CGATCTT7800.020.4771615
CCGTCGA550.00393015520.46117641
GGAGACG15550.020.2536667
GGGAGAC16100.019.561776
CGTGTGC14550.018.86265612
CGATCTC14300.016.5180685
CGTTTGC1104.6472785E-516.36075412
CGTCGTA2351.8189894E-1216.27372710
Produced by FastQC (version 0.11.2)