FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell43.352000000954e4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell43.352000000954e4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2481210
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC51800.027.1009333
CCGATCT51950.026.8927654
CGATCTG24750.026.542215
TTCCGAT53600.026.2747732
CGATCTA8200.026.0638875
CTTCCGA54200.026.0254171
CGATCTT9900.022.497255
GACGTGT14100.021.5399210
CGGGTAT855.0414255E-621.17388215
CGATCTC15450.019.9490185
GAGACGT16400.019.6165058
ACGTGTG15900.019.52591311
CGTGTGC18250.017.75125512
GGAGACG19650.017.6314337
AGACGTG17400.017.4547629
GTCTAAC1305.7966645E-717.3055761
ATTCGAC3200.016.8729381
GTATTAG8400.016.6051121
TCGACAC3150.016.4265633
GATCTGT10400.015.7913386
Produced by FastQC (version 0.11.2)