FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell42.352000000954ca.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell42.352000000954ca.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2119064
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA5700.031.1788655
TCCGATC37750.029.7962153
TTCCGAT38050.029.561292
CCGATCT38900.028.9731834
CTTCCGA39850.028.394711
CGATCTG19700.027.6348555
CGATCTT7600.024.5681535
ACGTGTG5250.020.13987711
GACGTGT5300.019.9498810
CGATCTC11900.018.7152795
CGAGCCG3450.017.60650615
GTATTAG6050.017.475931
ATACCGC3650.017.26057227
GAGACGT6650.017.2526828
GTATTAA7300.016.6405891
CGTCGAC952.8143046E-416.58393142
TAGACCG1104.6473026E-516.360835
GATCTGC10650.016.0535856
TCGACGG1004.159588E-415.75473444
CGTGTGC7150.015.73193812
Produced by FastQC (version 0.11.2)