FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell41.352000000954a1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell41.352000000954a1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2005108
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22140.11041799244728963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT33550.028.4967562
TCCGATC34100.027.9711593
CCGATCT34850.027.2400974
CGATCTT7200.027.1829515
CTTCCGA36250.026.3742371
CGATCTG17000.026.0691665
CGATCTA5350.023.547465
CGGTCGA706.98913E-419.2844120
GACGTGT6800.018.52677310
GAGACGT7800.018.1700368
CGATCTC11250.017.1971235
GTATTAG8800.017.1273881
CGATTAC1358.556035E-716.67302545
ACGTGTG7450.016.60878811
CATCGCG952.823198E-416.57715
CTTACCG1104.6469377E-516.3608999
TTGACGC1257.645793E-616.1968864
GTATAAC2101.07320375E-1016.0683381
ACGTCCG850.00255868515.88920443
ATCTGCG1855.638867E-915.8081657
Produced by FastQC (version 0.11.2)