FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell40.35200000095487.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell40.35200000095487.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2179987
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA22120.10146849499561236No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT36150.027.9402482
TCCGATC36500.027.7339553
CTTCCGA37000.027.6631661
CCGATCT37050.027.1401064
CGATCTG17850.025.456895
CGATCTA4950.023.6314495
CGATCTC11950.022.5894435
ATCTGCG1950.020.764917
CGATCTT8550.020.2589445
CGCCGTA1009.0726826E-720.24764625
GACGTGT7050.019.46403710
GAGACGT7550.018.7709318
AGACGTG7850.018.3401369
TAGTCGG1257.629238E-616.20071830
ACGTGTG8200.016.18565811
GTATATA8550.016.0492941
TAGCGCG2353.6379788E-1115.3159644
TCTTCCG15300.015.14390855
ACACCGT2101.8590072E-914.9968816
GTATAAA6950.014.5653151
Produced by FastQC (version 0.11.2)