FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell4.35200000095011.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell4.35200000095011.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1859442
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24420.1313297214970943No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC33950.032.0064663
TTCCGAT34600.031.535232
CTTCCGA34500.031.4301621
CCGATCT34500.031.3005874
CGATCTG17000.030.966875
CGATCTA5200.029.8521825
CGATCTC11600.025.0185475
ATCTGCG2650.023.770737
CGATCTT4800.022.965995
GGGTACG550.00393711220.45483832
CCGTAAT550.003940179520.45208714
CGACGTC1009.057512E-720.25083536
GACGTCG807.563806E-519.68831337
GATCTGG13050.018.963246
ACGTCGC851.203575E-418.5321738
ACCGTCG2500.017.9978398
GCATTCG3550.017.74530222
CGCCGTA1153.4540553E-617.60705425
ATACCGA901.868174E-417.4978986
GTATATA5900.017.1584951
Produced by FastQC (version 0.11.2)