FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell39.3520000009546d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell39.3520000009546d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2949672
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC51950.028.1922253
CTTCCGA53850.027.4477181
CCGATCT53050.027.4380234
TTCCGAT53550.027.3918912
CGATCTG25050.027.3023325
CGATCTA7100.026.9336075
CGATCTT11700.022.3051955
GACGTGT10800.020.83100310
GAGACGT12500.018.7179058
ACGTGTG11850.018.60551311
CGATCTC18150.017.6013365
AGACGTG13100.017.0019159
ACGACGG4150.016.263246
CGATTAC1301.1044713E-515.5815245
CGCCGTA1301.1080268E-515.57623725
CGTGTGC15300.015.29240612
TACGACG4300.015.1727225
GGAGACG16450.015.0439087
GTATTAA10650.014.7868281
CCGATCG6600.014.65769914
Produced by FastQC (version 0.11.2)