FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell38.35200000095444.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell38.35200000095444.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311808
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC45650.024.690473
CGATCTA9000.024.4971735
TTCCGAT46000.024.45372
CCGATCT46600.023.9940134
CGATCTG19850.023.9141185
CTTCCGA47250.023.9020041
GACGTGT12450.021.1421410
CGATCTT9250.020.9165045
GAGACGT13550.020.5880328
ACCGTCG2800.020.0869678
TACCGTC2750.018.8160117
AGACGTG13650.018.7890429
ACGTGTG13950.018.38497511
GGAGACG16100.017.74647
TATGCGA800.001712873716.8730535
CGTGTGC15750.016.71235812
CGATCTC14750.016.320155
GGGAGAC18850.015.992856
CGGTCAA2850.015.78867718
ATACCGT4100.015.3640816
Produced by FastQC (version 0.11.2)