FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell37.3520000009542a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell37.3520000009542a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3319974
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA54450.16400730849097012No Hit
TCTATAGGGTGATAGATTGGTCCAATTGGGTGTGAGGAGTTCAGTTATATG34270.10322369994463813No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA65750.030.2801651
TCCGATC65850.030.1663073
CCGATCT67350.029.193834
TTCCGAT68050.029.1906172
CGATCTG36250.029.105975
CGATCTA9650.028.674455
CGATCTC20000.023.2840025
CGATCTT8100.021.663415
ACGTCGA706.988674E-419.2851428
GATCTGG25600.018.8057696
GATCTGC17500.017.4830866
TACGACG6350.017.3595965
GATCTAC5850.016.9205346
TCTTCCG27400.016.8314135
CGACGGT6700.016.4527517
GATCTCG11850.016.326666
ACGACGG6800.016.2107986
GACGGTA6750.015.9975968
GTATTAA7800.015.5743471
GTCCTAT9450.015.2355761
Produced by FastQC (version 0.11.2)