FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell36.35200000095400.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell36.35200000095400.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933085
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA36760.12532879203978065No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC59950.031.1848283
CTTCCGA60300.031.1530571
TTCCGAT60450.030.8896732
CCGATCT61650.030.215434
CGATCTG32500.029.8349595
CGATCTA8600.027.72935
CGATCTC18250.023.9155
CGATCTT10000.022.4973585
ATCTGCG4900.022.4973567
TATCCCG1251.7724233E-819.7976765
GATCTGC17850.018.0230926
GATCTCG9900.017.9524366
TCTTCCG22500.017.6979225
GTATTAG10500.017.5693651
GACGTGT5100.017.20386310
GATCTGG23350.017.150026
TTAACGG4300.016.7453635
TAACGGC4700.016.27773736
GTATATA7750.015.6755781
CGGTACC4600.015.65727645
Produced by FastQC (version 0.11.2)