FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell35.352000000953f2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell35.352000000953f2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2713847
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA9900.029.0870865
TCCGATC58550.026.473893
CCGATCT58800.026.246554
TTCCGAT59400.026.1708012
CTTCCGA60950.025.542171
CGATCTG25950.025.1412055
CGATCTT11700.024.4198675
GAGACGT19050.021.9656188
GACGTGT18950.021.84409310
ACGTGTG19150.021.14604611
AGACGTG20100.019.9227549
GGGAGAC23150.019.338716
TATACGT600.00651767118.7475365
GGAGACG23600.018.6839857
ACCGTCG4150.018.4313138
CTATACG1102.261122E-618.4066724
CGTGTGC22250.018.30130412
CGATCTC18000.018.1226185
CGTCGTA4300.017.7883610
TACCGTC4500.017.49777
Produced by FastQC (version 0.11.2)