FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell34.352000000953cb.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell34.352000000953cb.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2111259
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG21500.028.3569285
TCCGATC48300.027.9934023
TTCCGAT48550.027.8029172
CCGATCT48900.027.6499254
CTTCCGA49550.027.4681721
CGATCTA8300.026.020855
CGATCTT9350.023.0987225
GACGTGT11000.021.67962810
GAGACGT11950.020.5204528
ACGTGTG11700.020.19026811
CGATCTC16350.019.263655
GGGAGAC14350.018.656216
GGAGACG14700.018.3650557
TATACCG901.868392E-417.4978185
AGACGTG13350.017.3573869
CGTGTGC13650.017.14112512
GGCGTTA2301.8189894E-1216.6342742
ATTCGTA6550.016.48766524
GATCTGG15150.016.4830916
GCATTCG6650.016.23972922
Produced by FastQC (version 0.11.2)