Basic Statistics
Measure | Value |
---|---|
Filename | HT5LJBCXY_l02n02_inf_cell33.352000000953a2.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 142216 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 52 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 2064 | 1.4513134949654047 | No Hit |
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 291 | 0.2046183270518085 | No Hit |
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 245 | 0.17227316195083534 | No Hit |
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT | 190 | 0.13359959498228047 | Illumina Multiplexing PCR Primer 2.01 (95% over 21bp) |
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT | 158 | 0.11109861056421218 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGAGGAC | 20 | 7.002169E-4 | 45.013367 | 41 |
GCCTCTG | 25 | 3.866734E-5 | 45.013367 | 40 |
CTCTGTT | 25 | 3.866734E-5 | 45.013367 | 42 |
TTTCGGA | 30 | 2.1528704E-6 | 44.99754 | 1 |
TGTGATA | 30 | 2.1528704E-6 | 44.99754 | 34 |
TAACATG | 20 | 7.014319E-4 | 44.99754 | 29 |
GGGGTAC | 20 | 7.014319E-4 | 44.99754 | 6 |
TCCAATC | 20 | 7.014319E-4 | 44.99754 | 22 |
GGACACT | 20 | 7.014319E-4 | 44.99754 | 7 |
GTTCTGC | 25 | 3.8747792E-5 | 44.997536 | 25 |
TCTAGCC | 25 | 3.8747792E-5 | 44.997536 | 27 |
GTAGACA | 25 | 3.8747792E-5 | 44.997536 | 36 |
ACCCGTT | 25 | 3.8747792E-5 | 44.997536 | 21 |
TCGAGGG | 25 | 3.8747792E-5 | 44.997536 | 2 |
CTGTGAT | 25 | 3.8747792E-5 | 44.997536 | 33 |
CTGGTAT | 45 | 1.9088475E-8 | 39.99781 | 33 |
CTGGCCG | 35 | 6.212089E-6 | 38.569317 | 28 |
CTTCCGT | 160 | 0.0 | 37.966675 | 20 |
ATCTCCT | 95 | 0.0 | 37.892666 | 26 |
ACACCCG | 30 | 1.13526396E-4 | 37.497948 | 19 |