FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell33.352000000953a2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell33.352000000953a2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences142216
Sequences flagged as poor quality0
Sequence length51
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA20641.4513134949654047No Hit
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2910.2046183270518085No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.17227316195083534No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT1900.13359959498228047Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1580.11109861056421218No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGAC207.002169E-445.01336741
GCCTCTG253.866734E-545.01336740
CTCTGTT253.866734E-545.01336742
TTTCGGA302.1528704E-644.997541
TGTGATA302.1528704E-644.9975434
TAACATG207.014319E-444.9975429
GGGGTAC207.014319E-444.997546
TCCAATC207.014319E-444.9975422
GGACACT207.014319E-444.997547
GTTCTGC253.8747792E-544.99753625
TCTAGCC253.8747792E-544.99753627
GTAGACA253.8747792E-544.99753636
ACCCGTT253.8747792E-544.99753621
TCGAGGG253.8747792E-544.9975362
CTGTGAT253.8747792E-544.99753633
CTGGTAT451.9088475E-839.9978133
CTGGCCG356.212089E-638.56931728
CTTCCGT1600.037.96667520
ATCTCCT950.037.89266626
ACACCCG301.13526396E-437.49794819