HT5LJBCXY_l02n02_inf_cell32.35200000095388.fastq.gz FastQC Report

Basic Statistics

Measure	Value
Filename	HT5LJBCXY_l02n02_inf_cell32.35200000095388.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	148732
Sequences flagged as poor quality	0
Sequence length	51
%GC	53

Per base quality graph

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1215	0.8169055751284189	No Hit
CGTCTAGATCGGAAGAGCACACGTCTAGATCGGAAGAGCACACGTCTAGAT	236	0.15867466315251594	Illumina Multiplexing PCR Primer 2.01 (95% over 21bp)
GTGCTCTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	186	0.1250571497727456	No Hit
CTTCCGATCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	182	0.12236774870236398	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTGGAC	20	6.9930806E-4	45.02658	43
TGCTCCC	20	6.9930806E-4	45.02658	45
GCTCGTT	20	6.9930806E-4	45.02658	42
CGGTGTC	20	7.004687E-4	45.011433	40
ATCTCTA	30	2.1539472E-6	44.9963	26
TGTGGGC	25	3.8762686E-5	44.9963	32
ATCCGGT	30	2.1539472E-6	44.9963	26
ATCCGGC	25	3.8762686E-5	44.9963	26
ACGTTTT	45	1.9103027E-8	39.99671	11
GCGCCGT	40	3.4334334E-7	39.371765	37
TGGGAGT	40	3.4334334E-7	39.371765	5
TCTCGTC	115	0.0	39.12722	27
GATCCGG	65	9.094947E-12	38.07379	25
GCCGCTC	30	1.1334503E-4	37.50953	39
CTAAGGG	30	1.13569804E-4	37.496918	2
GGGCGTC	60	1.5279511E-10	37.496918	35
GAGCGGT	30	1.13569804E-4	37.496918	37
GCGTGCA	30	1.13569804E-4	37.496918	13
GTTATAC	30	1.13569804E-4	37.496918	35
CTCGTTC	25	0.0020951412	36.021267	43