FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell31.3520000009536e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell31.3520000009536e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2012520
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23390.11622244747878281No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGCG300.005151378529.99617416
CGATCTG17550.027.814895
TTCCGAT35950.027.782982
TCCGATC35900.027.6963523
CCGATCT36250.027.2427714
CTTCCGA37350.026.8018111
CGATCTA4850.026.4379545
GAGACGT8700.023.2711588
GACGTGT8750.021.33854510
CGATCTT7700.021.3268595
ACGTGTG8700.020.94404211
AGACGTG9550.019.7865779
CGATCTC11100.019.4555265
CAATCGA706.976777E-419.28948438
GGGAGAC12050.019.228486
GGAGACG11400.018.7462127
CGTGTGC10550.017.69784512
CGGGAGA3450.017.6051375
TCGAGTA901.8612889E-417.50646841
TATAACG901.8694422E-417.4964642
Produced by FastQC (version 0.11.2)