FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell30.35200000095345.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell30.35200000095345.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432365
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC45900.028.5721243
CGATCTA8750.028.2794745
TTCCGAT46650.028.2092082
CTTCCGA47350.028.1241531
CCGATCT46450.027.991674
CGATCTG20250.027.2162155
GTCGACG903.2762182E-722.50984443
CGATCTT9450.022.3760155
CGATCTC15100.019.8135075
GACGTGT9550.019.78661510
CGTCGAC1051.4414436E-619.29335842
GACGCGT851.2026246E-418.53446839
GAGACGT10250.018.4349568
TCGACGG1002.0404705E-518.00787444
CGGTTAA750.001107758418.00713342
CCGGTTA750.001108217218.00602141
TCTAACG750.001112353417.9960298
ATCTGCG2900.017.8408897
ACGTGTG10150.017.73080611
CGGGTAT1153.457788E-617.60553415
Produced by FastQC (version 0.11.2)