FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell3.35200000094ff9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell3.35200000094ff9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1171292
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA16140.13779655286640735No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGG12470.10646363161363691No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGATCG300.005151430629.99507715
CTTCCGA17850.029.7430171
TCCGATC18400.029.0984843
TTCCGAT18550.028.8631862
CCGATCT18500.028.3331874
CGATCTG7800.028.2645935
CGATCTA3500.027.6383195
CGATCTC6200.022.859155
GGTACCT7150.021.395099
TAAACGG855.0357758E-621.17480317
CGTCCGA550.003932538420.45817437
ACGTCCG908.232746E-620.00525743
CGTCGTA706.991296E-419.28254910
TCTTCCG7550.019.0697175
CGATTTG1555.329639E-1018.87592943
GCCGCGA600.00649909318.7557345
TAGGGGT4200.018.7469234
GTTGATC8550.018.6827417
GTATTAG4800.018.278251
GGGTACC8250.018.2697288
Produced by FastQC (version 0.11.2)