FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell29.3520000009532b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell29.3520000009532b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983854
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27230.1372580845163001No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC36250.030.0974453
TTCCGAT36450.029.93232
CTTCCGA36850.029.6073911
CGATCTG17700.029.3592915
CCGATCT37400.028.9313984
CGATCTA6000.025.8704935
CGATCTT7200.023.4334185
CGATCTC11400.021.3120775
GACGTGT6450.020.92711310
GAGACGT7500.019.4966038
CGGTACC1656.002665E-1119.10058245
ACGTGTG6950.019.09787411
CGGTCGA750.001111156317.9986820
GCGTCAA1002.049146E-517.99867826
ATCGCTA1002.0504793E-517.99731810
CCGTTTA1153.4561308E-617.60607313
AGACGTG7650.017.0558539
GGAGACG9400.016.7524017
TATTACG952.8249418E-416.5756424
CGGTACT3700.016.42268238
Produced by FastQC (version 0.11.2)