FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell28.35200000095301.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell28.35200000095301.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2208817
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC40250.028.9526623
TTCCGAT40500.028.7739432
CCGATCT40550.028.6829814
CGATCTA6500.028.380835
CTTCCGA41800.028.0405181
CGATCTG19900.027.8103625
CGATCTT7950.024.3363785
TCGACGG651.5210766E-524.23796544
CGTCGAC754.5985886E-521.00385742
CGTTATA654.2437526E-420.7664622
GACGTGT6000.020.24821710
CGATCTC12450.019.1540745
GAGACGT6650.018.606548
GTCGACG851.2023391E-418.53491443
CCGTCGA851.20380115E-418.53197741
GGCGTTA2900.017.84810442
GTATTAA6950.016.1848931
ACGTGTG6850.016.09347311
GCGGAAT850.00256651315.88203419
GTATATA8350.015.6266591
Produced by FastQC (version 0.11.2)