FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell27.352000000952e6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell27.352000000952e6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2526137
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31400.12430046351405329No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC45150.027.3052583
CTTCCGA45500.027.094681
TTCCGAT45600.027.0851332
CGATCTG22250.026.996335
CCGATCT45550.026.719754
CGATCTA7450.026.5735685
CCGTCGA651.5220894E-524.2361141
CGATCTT8800.022.2412955
CGCCGTA1101.0006261E-720.45257425
GTCGACG807.543278E-519.69574243
TCTTCCG19650.018.432615
TTAACGG3100.018.14700535
TCGACGG1002.0408537E-518.00753444
TCGTCTA750.001111294617.99862323
GACGTGT6900.017.93234610
CGGTACC3050.017.71232845
CGATCTC12800.017.5757375
GAGACGT7500.016.7977168
ACGTGTG7500.016.19779811
ATCTGCG3000.015.7478597
Produced by FastQC (version 0.11.2)