FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell26.352000000952cc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell26.352000000952cc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2103110
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA27060.1286665937587668No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC36300.028.012373
CGATCTA6150.027.8007495
CCGATCT36900.027.4959164
CTTCCGA37150.027.4319951
TTCCGAT37200.027.3346522
CGATCTG16300.025.5330185
CGATCTT7600.023.3846865
GACGTGT6300.022.49665810
CGATCTC12000.021.1843535
GAGACGT7100.020.9123888
ACGTGTG6750.019.99703211
ACGTCCG1251.758417E-819.8107243
CGGTCGA600.00651515218.74855220
AGACGTG7800.017.5935429
GGAGACG9450.016.6641927
CGTGTGC8250.016.63428912
CGGGTAT952.8234354E-416.5768815
GATCTCG6050.016.3612086
GGGAGAC10050.016.117016
TTAGACG1703.4022378E-815.88528239
Produced by FastQC (version 0.11.2)