FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell25.352000000952a3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell25.352000000952a3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2071864
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA23020.1111076788823977No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC39300.027.533813
CGATCTG19450.027.2964535
CCGATCT39350.027.2701444
CTTCCGA40150.027.0623131
TTCCGAT40850.026.5992182
CGATCTA6900.025.1047235
TCGACGG955.512484E-721.32528344
CGATCTC12350.020.7659445
CGATCTT5750.020.3446085
GTATTAG8600.019.6185151
CGTCGAC1002.0417889E-518.00627942
GTATATA7800.016.7277181
TCTTCCG18100.016.6548235
ACGTGTG5850.016.53584711
GTCGACG1104.6227426E-516.37092643
ACGTAGA1104.6428548E-516.36262724
GAGACGT6350.016.2966358
TCTAATA6500.016.2666572
TAAACGG2950.016.01441414
TATAGAC2009.313226E-1015.7475093
Produced by FastQC (version 0.11.2)