FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell23.3520000009526f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell23.3520000009526f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2648226
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA37280.14077348383408364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC48450.025.90833
TTCCGAT49050.025.6372412
CCGATCT49000.025.3420374
CTTCCGA50250.025.2936131
CGATCTG21000.025.17375
CGATCTT9800.025.020675
CGATCTA8050.023.473725
GAGACGT15250.021.3893058
GACGTGT15350.020.22448210
GGGAGAC18500.018.9693036
GGAGACG18200.018.9111797
ACGTGTG16450.018.7353311
AGACGTG16400.018.517769
GTATTAG10850.018.0379851
CGGTACC4250.016.95166245
CGTGTGC18350.016.79543312
CGATCTC17500.016.4539595
GGCGTTA2750.016.37252842
GCGTTAT3150.015.72401343
TTAACGG4900.015.613646535
Produced by FastQC (version 0.11.2)