FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell22.35200000095253.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell22.35200000095253.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135891
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA31660.1482285378795079No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC52750.027.21013
TTCCGAT53400.027.0474112
CCGATCT53000.026.9968534
GAGACGT15000.026.696898
CGATCTG24900.026.472825
GACGTGT14300.026.2731610
CTTCCGA55450.026.209751
CGATCTA8950.026.1422045
CGATCTT8850.024.1497295
ACGTGTG15250.023.7513311
AGACGTG15900.023.6293229
GGAGACG17900.023.3771637
GGGAGAC19300.022.1476786
CGTGTGC17950.021.05604212
CGATCTC16200.020.1365415
CTAGCCG600.00651666718.7478169
ACGTTTG2050.018.65636311
GTATATA6750.017.6646081
GATCTGG13900.017.6418296
ATCACGC1551.0921212E-817.41854928
Produced by FastQC (version 0.11.2)