FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell21.3520000009522c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell21.3520000009522c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2228275
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTA8600.027.9890635
TCCGATC51750.027.2993493
CTTCCGA51950.027.1076471
TTCCGAT52000.027.038322
CCGATCT51750.026.9081174
CGATCTG23550.026.5556495
CGATCTT9200.023.7184855
GAGACGT13450.020.9069528
TGCGACG550.00394083220.4517210
GACGTGT13300.020.128810
ACGTGTG13850.019.49189211
CGATCTC16800.018.8804725
AGACGTG14800.017.9359079
GGGAGAC18200.017.6753376
GGAGACG17550.017.5608887
GGCGTTA1701.8244464E-917.21200442
GATCTAC6350.016.6504966
GATCTGG14700.015.7624216
ACGAATC1004.1650713E-415.75206637
CGTGTGC17600.015.59443612
Produced by FastQC (version 0.11.2)