FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell20.35200000095202.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell20.35200000095202.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1723945
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT36550.027.4503752
TCCGATC36200.027.2807773
CGATCTG17350.026.8393295
CCGATCT36900.026.702294
CTTCCGA38100.026.1564921
CGATCTA5500.025.3588645
CGATCTT5800.021.3321915
TAAGTCG654.2448047E-420.765325
CGATCTC12500.020.6961045
GAGACGT8850.020.5893448
GACGTGT8600.020.14214310
AGACGTG8650.019.7650669
ACGTGTG8350.019.1286911
ATCTGCG2450.018.363897
ATAATAC6050.017.476053
GGGAGAC11200.017.072686
CGTAAAC800.001713470316.8718243
GGAGACG11400.016.7731597
GATCTAG3700.016.4158296
TATGCGC850.00256610515.88212929
Produced by FastQC (version 0.11.2)