FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell2.35200000094fdf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell2.35200000094fdf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1002664
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA15250.1520948194011154No Hit
CTTTTAGCTGTTCTTAGGTAGCTCGTCTGGTTTCGGGGGTCTTAGCTTTGG11930.11898302921018408No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC17550.028.715023
CTTCCGA17600.028.3777891
TTCCGAT17900.028.1535552
CCGATCT18050.027.9195924
CGATCTG8800.026.8438555
CGATCTA3300.026.5881985
CGGGTAC1500.023.9975537
TTTCGGG2650.022.92447731
GGTACCT5900.022.4977079
ACGGGTA702.7046766E-522.4977076
CCGGGTA855.0355957E-621.1743136
CGAACTA754.6037276E-520.99890729
CGATCTC5150.020.9687355
CGATCTT3550.020.9133635
TCTTCCG7350.019.8959315
TTCGGGG2950.019.83046532
GGGGTTA2950.019.8284876
GTTTCGG3050.019.17932930
AGTGCGG600.006513762818.74808910
GGCGTTA851.2005878E-418.53676642
Produced by FastQC (version 0.11.2)