FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell19.352000000951e7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell19.352000000951e7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1908525
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATCTG17300.027.8274295
TCCGATC37100.027.6500763
TTCCGAT37600.027.3422182
CCGATCT37850.027.0427514
CTTCCGA39400.026.1501771
CGATCTA5350.025.2291625
CGATCTT6800.024.811775
GAGACGT10450.022.6036428
GACGTGT10100.022.05111910
ACGTGTG10800.020.41357611
GGAGACG12350.020.0369267
CGATCTC12900.018.8338665
ACCGTAC1202.5020927E-718.74765217
GGGAGAC13750.018.6512346
AGACGTG12100.018.4058239
CGTGTGC13750.017.34282712
AACCGTA1305.7951183E-717.30552716
GTATTAA7400.017.0240041
GATCTGC9650.017.0177046
GAATCGG1104.641947E-516.36287522
Produced by FastQC (version 0.11.2)