FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell18.352000000951cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell18.352000000951cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2178463
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA24540.11264822950860308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC42200.025.2687473
TTCCGAT42650.025.0548842
CCGATCT43200.024.6317484
CTTCCGA43350.024.5984121
CGATCTA7150.024.5417985
CGATCTG18650.024.3663435
GAGACGT11150.022.5975328
GACGTGT11050.021.9881810
ACGTGTG10750.021.13687111
GGGAGAC13750.019.6334386
GGAGACG13900.019.4215687
CGATCTC14900.019.3259815
AGACGTG12800.018.8057929
CGCACGT851.2030541E-418.53345940
CGATCTT8400.018.479395
CGTGTGC13500.017.49779512
GTATTAA6750.016.9974691
TCTAACG800.001713215616.8724862
CGGTACC3150.015.71988245
CGTAGCG2051.3205863E-915.3642715
Produced by FastQC (version 0.11.2)