FastQCFastQC Report
Thu 20 Jul 2017
HT5LJBCXY_l02n02_inf_cell17.352000000951b0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHT5LJBCXY_l02n02_inf_cell17.352000000951b0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2141381
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTATACCTTCTGCATAATGAATTAACTAGAAATAACTTTGCAAGGAGA25860.12076318973596946No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT42400.025.415152
TCCGATC42200.025.322363
CTTCCGA43300.025.0421731
CGATCTA6200.025.0368925
CCGATCT43050.024.7701264
CGATCTG19650.023.4700665
CGATCTT8900.022.2441445
GACGTGT12900.021.62494510
GAGACGT14750.021.2004858
ACGTGTG14050.020.65601511
GACGTTT1603.8198777E-1119.68480310
GGAGACG16800.019.2830727
GGGAGAC16800.019.2830726
CGATCTC13350.019.0423345
AGACGTG14950.018.9606139
ATTAGCG600.00651217118.7500636
TTAACGG3650.017.26032635
CGTGTGC16200.017.22026612
TATAGCG800.001713065416.8726885
GTATTAG8800.016.6166531
Produced by FastQC (version 0.11.2)